Introduction to Editing the Mouse Genome

Lydia has over 25 years of experience of mouse genome engineering, with expertise in both classical and genome editing methods. She introduced the use of the CRISPR/Cas9 system at the MLC in 2013 and has employed these tools in both a high throughput process for the generation of null mice and a bespoke activity for the generation of point mutations and more complex mouse models. Her team has also defined quality control criteria and reliable protocols to validate mutants generated using CRISPR/Cas9 and classical tools. Lydia manages genome engineering activities and the development of new in vitro service platforms at the MLC. She has also helped to write the international guidelines for the reporting of the genetics of laboratory animals.

Through her involvement with the Advance Training Centre, Lydia brings extensive experience in molecular biology, helping to support the community in designing, creating and using genetically altered animals.