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Genetics

Genome Engineering and Validation by Long Read Sequencing

An interactive introduction to genome engineering validation at Advance, Harwell. Learn how long-read sequencing technologies can be used to validate engineered alleles, analyse sequencing data and optimise genome editing workflows.

  • Genetics
  • 1 day
Tutor
Lydia Teboul
Availability
Register interest
Cost
£450
Duration
1 day

Course Overview

This interactive training session explores the use of long read sequencing (LRS) technologies for validating engineered alleles in both cellular and embryonic genome engineering contexts. Participants will learn the principles of genome editing design, how LRS compares to traditional validation methods, and how to select the appropriate sequencing modality for different allele types and experimental materials.

Who is this for?

This workshop is designed for Early-career researchers and Experienced technicians

  • seeking foundational knowledge in genome editing and sequencing technologies.
  • aiming to refine protocols, troubleshoot workflows, and deepen data analysis skills.

Learning Outcomes:

  • Understand genome editing principles and allele design strategies
  • Learn the rationale for using LRS in validation workflows
  • Gain confidence in interpreting sequencing data and alignment files
  • Update and optimize genome editing and sequencing protocols
  • Compare LRS platforms and outsourcing options
  • Deepen skills in troubleshooting and data analysis using .bam files

Workshop Agenda:

1. Genome Editing Principles & Allele Design

  • Overview of genome editing technologies (e.g., CRISPR/Cas9, base editing)
  • Strategies for designing engineered alleles
  • Design implications for validation
  • Considerations for validation in cells and animals

2. Targeted Sequencing Modalities

  • Why use LRS instead of Sanger?
  • Overview of LRS platforms and their relevance to allele validation
  • Choosing the right approach for your allele and material

3. Experimental Workflows

  • Sample preparation and library construction
  • Platform-specific considerations (e.g., ONT, PacBio)
  • Adaptations for sample types (cell culture, founder animals, subsequent generation)

4. Step-by-Step Protocols

  • Practical guidance for generating high-quality data

5. Data Analysis & Software Tools

  • Principles of LRS data interpretation
  • Overview of analysis software
  • Guided exercises with real datasets

6. Deep Dive into Alignment Files (.bam)

  • How to read and interpret .bam files
  • Examples from in-house and outsourced sequencing
  • Platform-specific nuances and troubleshooting

Includes

  • A primer on genome editing strategies and allele design
  • Protocols for sequencing and data generation
  • Software tools for data analysis
  • Hands-on exercises with real datasets
  • Case studies from in-house and outsourced sequencing projects

Course details

Where

This course takes place at Advance, Harwell. You will be working in dedicated training spaces designed for practical scientific learning.

Format

This is a 1 day practical course built around active participation, demonstrations, and supervised technical work.

Level

Suitable for learners who want to translate scientific knowledge into stronger practical capability.

Availability

Register interest

Cost

Course fee: £450. Contact the team if you need help exploring funding or team bookings.

Tutors

Meet Your Tutor

This course is taught by active practitioners who bring current methods, practical judgement, and real laboratory context into every session.

Lydia Teboul

Lydia Teboul

Head of Molecular and Cellular Biology at the Mary Lyon Centre at MRC Harwell

PhD in Molecular and Cellular Biology, University of Nice

Lydia has over 25 years of experience of mouse genome engineering, with expertise in both classical and genome editing methods. She introduced the use of the CRISPR/Cas9 system at the MLC in 2013 and has employed these tools in both a high throughput process for the generation of null mice and a bespoke activity for the generation of point mutations and more complex mouse models. Her team has also defined quality control criteria and reliable protocols to validate mutants generated using CRISPR/Cas9 and classical tools. Lydia manages genome engineering activities and the development of new in vitro service platforms at the MLC. She has also helped to write the international guidelines for the reporting of the genetics of laboratory animals.

Through her involvement with the Advance Training Centre, Lydia brings extensive experience in molecular biology, helping to support the community in designing, creating and using genetically altered animals.

  • Genetics
  • Transgenics

What You Will Take Away

The course is designed to give learners practical outcomes they can carry back into their own work immediately.

The whole course was very interesting and engaging… particularly impressive given the complexity.

Understand genome editing principles and allele design strategies

Gain confidence in interpreting sequencing data and alignment files

Deepen skills in troubleshooting and data analysis using .bam files

In Their Own Words

General

“The whole course was very interesting and engaging… particularly impressive given the complexity.”

General

“The best thing about the course was the depth of knowledge and friendliness of the training team.”

General

“The worked examples and tasks were really helpful for the data analysis section and made me feel confident that I could give this a go myself.”

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Questions

What should I bring?

Bring a notebook and any device you regularly use for work. We provide the equipment and materials needed for the practical sessions.

Is prior experience required?

The course is designed for learners who want to build or deepen practical capability. If you are unsure about your level, contact us and we can advise.

Do you offer group bookings?

Yes. We can discuss group bookings, scheduling, and practical requirements for teams or organisations.

Ready to Advance Your Practice?

Secure your place or register your interest today